A microtiter plate was coated over night with MCA2112 at concentration of 5μg/ml in BUF030A. After washing with BUF031A and blocking with BUF032A samples were detected using MCA1783B antibody at 5μg/ml followed by incubation with streptavidin:HRP conjugate STAR5B at 1/1000. Finally, BUF056A was used as substrate. Data is presented as the mean of three measurements.
A microtiter plate was coated over night with MCA2112 at concentration of 5μg/ml in. After washing with and blocking with samples were detected using antibody at 5μg/ml followed by incubation with streptavidin:HRP conjugate at 1/1000. Finally was used as substrate. Data is presented as the mean of three measurements.
Supplier Page from Bio-Rad for 10x ELISA WASH BUFFER